What is D-luciferin used for?
D-Luciferin (D-(-)-Luciferin) is the substrate of luciferases that catalyze the production of light in bioluminescent insects. D-luciferin is the natural substrate of the enzyme luciferase (Luc), that catalyzes the production of the typical yellowgreen light of fireflies.
Is luciferin an acid?
Ent-Photinus luciferin is a 1,3-thiazolemonocarboxylic acid consisting of 3,5-dihydrothiophene-4-carboxylic acid having a 6-hydroxybenzothiazol-2-yl group at the 2-position. It has a role as a luciferin and an animal metabolite.
What is luciferin chemical?
Luciferin (from the Latin lucifer, “light-bearer”) is a generic term for the light-emitting compound found in organisms that generate bioluminescence. Luciferins typically undergo an enzyme-catalyzed reaction with molecular oxygen.
How do you inject luciferin?
(Luciferin is typically administered either intraperitoneally or intravenously.) Example: Inject with 10 µL of Luciferin stock solution per gram of body weight (normally ~200 µL for a 20 g mouse for a standard 150 mg/kg injection). 5. Wait 10-20 minutes before imaging for maximum luciferase signal plateau.
What is the difference between luciferase and luciferin?
One is a luciferin, or a light-producing substance. The other is a luciferase, or an enzyme that catalyzes the reaction. In some cases, the luciferin is a protein known as a photoprotein, and the light-making process requires a charged ion to activate the reaction.
How do you make D-Luciferin?
1. Prepare a 200X Luciferin stock solution (30 mg/ml) in sterile water. Note: One can either reconstitute the entire 1.0 g of D-Luciferin in 33.3 mL of sterile water to make the 30 mg/mL (200x) stock solution, or reconstitute the quantity of D-Luciferin necessary for an individual experiment.
What is an example of luciferin?
Definition of luciferin : any of various organic substances in luminescent organisms (such as fireflies) that upon oxidation produce a virtually heatless light.
Is luciferin naturally occurring?
Luciferase is an enzyme that catalyzes a light-producing biochemical reaction when it is in the presence of oxygen and a naturally occurring substrate called luciferin.
What type of enzyme is luciferase?
Luciferases are proteins with enzymatic activity that, in the presence of ATP, oxygen, and the appropriate substrate (typically luciferin), catalyze the oxidation of the substrate in a reaction that results in the emission of a photon.
How do you dissolve D-Luciferin?
Prepare a 200X luciferin stock solution (30 mg/ml) in sterile water. Mix gently by inversion until luciferin is completely dissolved. Use immediately, or aliquot and freeze at -20°C for future use.
What is the chemical formula for luciferin?
C11H8N2O3S2Firefly luciferin / Formula
Is luciferin soluble in water?
Answer: The Free Acid form of Luciferin will not dissolve in water unless a dilute base such as NaOH or KOH is added to adjust the pH. However, it is soluble in Methanol at 10 mg/ml or in DMSO at 50 mg/ml.