What is Nextera XT?
The Nextera XT DNA Library Preparation Kit enables researchers to prepare sequencing-ready libraries for small genomes (bacteria, archaea, and viruses), PCR amplicons, and plasmids in 90 minutes, with only 15 minutes of hands-on time.
What is nextera Flex?
Nextera™ DNA Flex Library Preparation Kit. A fast, integrated workflow for a wide range of applications from human whole-genome sequencing to amplicons, plasmids, and microbial species. For Research Use Only. Not for use in diagnostic procedures.
What is Tagmentation by Tn5?
Tagmentation-based library construction, using the Tn5 transposase, is efficient for generating sequencing libraries but currently relies on undisclosed reagents, which severely limits development of novel applications and the execution of large-scale projects.
What is P5 and P7?
P5 and P7 are names given by Illumina to the oligo sequences that bind to the flow cell. Source publication. Multiplexed Illumina sequencing libraries from picogram quantities of DNA.
What are dual index primers?
The Multiplexing Unique Dual Index Primers contains primer mix for multiplexing samples for Next Generation Sequencing (NGS) on the illumina platform. Multiplexing of NGS samples will reduce sequencing costs by pooling multiple NGS libraries into a single flow cell lane.
Does Tn5 cut DNA?
Tn5 transposases are versatile enzymes that randomly cut DNA and simultaneously insert transposons (adapters) into DNA, and the resulting fragments are ready for PCR amplification and sequencing.
What is MiSeq?
The MiSeq is an integrated instrument that performs clonal amplification, sequencing, and data analysis (base calling, alignment, variant calling, and reporting) in a single run.
What is single index and dual index?
Single-indexed libraries—Adds up to 48 unique 6-base Index 1 (i7) sequences to generate up to 48 uniquely tagged libraries. ▶ Dual-indexed libraries—Adds up to 24 unique 8-base Index 1 (i7) sequences and up to 16 unique 8-base Index 2 (i5) sequences to generate up to 384 uniquely tagged libraries.
What are Udi adapters?
sparQ UDI Adapters (1-96) are unique dual-indexed adapters designed to be used on Illumina sequencing platforms. It is compatible with both DNA and RNA libraries. The use of UDI’s minimizes index hopping events while also enabling greater multiplexing on flow cells. Details.