How does CFSE staining work?
Unlike stains that label the lipid membrane of cells, CellTrace™ CFSE dye easily crosses the plasma membrane and covalently binds inside cells where the stable, well-retained fluorescent dye is designed to provide a consistent signal, even after several days in a cell culture environment.
What is cell trace?
CellTrace™ Violet Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry. • Superior performance—bright, single-peak staining enables visualization of multiple generations.
How does CellTrace violet work?
CellTrace™ Violet easily diffuses into cells where it is cleaved by intracellular esterases to yield a highly fluorescent compound. This compound covalently binds to intracellular amines, resulting in stable, well-retained fluorescent staining that can be fixed with aldehyde fixatives.
Does CFSE bind DNA?
CFSE is cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules, notably, to intracellular lysine residues and other amine sources. Due to this covalent coupling reaction, fluorescent CFSE can be retained within cells for extremely long periods….Carboxyfluorescein succinimidyl ester.
| Names | |
|---|---|
| Molar mass | 473.393 g·mol−1 |
How do cell tracker dyes work?
CellTracker dyes are non-toxic at working concentrations. The simplest processes for monitoring cell movement and location use tracking probes that pass through the membrane into the cell and become membrane-impermeant after loading.
What is PKH26?
PKH26 is a red‐fluorescent dye. This lipophilic membrane dye is widely used to determine cell proliferation in heterogeneous cells by flow cytometry.
Does cell Tracker stain dead cells?
Unlike CellTracker™ and CellTrace™ probes described below, the calcein AM probes are retained only in live cells with esterase activity; they do not label dead or dying cells with compromised membranes.
What is cell mask?
The CellMask™ Plasma Membrane Stains allow fast and uniform labeling of the plasma membrane without the cell-type differences exhibited by lectins. These stains may be used as a segmentation tool for HCS (high-content screening), as well as to stain cellular plasma membranes for standard fluorescence microscopy.
What is PKH67?
PKH67 labelling method is a powerful tool for cell proliferation assessment in patients with AML, even in cells treated by various drugs.
What is Invitrogen?
Invitrogen creates products for cellular, protein and molecular biology research that allow scientists to innovate. Shop Invitrogen products at Fisher Scientific. Invitrogen | Fisher Scientific Fisher Scientific Fisher Healthcare Fisher Science Education Sign Up for Email Help & Support Center › 0 No offer available
Why choose Invitrogen™ megax™ cells?
Invitrogen™ MegaX DH10B T1RElectrocomp™ Cells Highest-efficiency electrocompetent cells available Invitrogen™ cell and molecular biology products range from do-it-yourself reagents and optimized kits to connected instruments, all working together holistically to enable scientists to do what they do best—only better.
What is Invitrogen Evos XL core imaging system?
Invitrogen™ EVOS™ XL Core Configured Cell Imager The EVOS XL Core Imaging System is a digital transmitted-light inverted imaging system for cell and tissue culture applications and routine cell maintenance. IgG (H+L) Highly Cross-Adsorbed Donkey anti-Mouse, Alexa Fluor™ 488, Invitrogen™
What kind of imaging system does Invitrogen use?
Invitrogen™ EVOS™ XL Core Configured Cell Imager The EVOS XL Core Imaging System is a digital transmitted-light inverted imaging system for cell and tissue culture applications and routine cell maintenance. IgG (H+L) Highly Cross-Adsorbed Donkey anti-Mouse, Alexa Fluor™ 488, Invitrogen™ Donkey Polyclonal Secondary Antibody