Is Burkholderia cepacia oxidase positive?
All of the B. cepacia and B. gladioli strains were positive in the oxidase assay. The results of growth analyses with selective agar plates are shown in Table
Is Burkholderia cepacia catalase positive?
Burkholderia cepacia (B. cepacia), formerly known as Pseudomonas cepacia, is an aerobic, catalase-positive, gram-negative rod that was first isolated in 1950 as the responsible agricultural pest for onion skin rot.
What are the characteristics of Burkholderia cepacia?
They are rod-shaped, free-living, motile Gram- negative bacteria ranging from 1.6- 3.2 μm. They have been found to possess multitrichous polar flagella as well as pili used for attachment. Burkholderia cepacia can be found in soil, water, and infected plants, animals, and humans (1).
Is Burkholderia cepacia oxidase positive or negative?
Biochemical Test of Burkholderia cepacia
| Basic Characteristics | Properties (Burkholderia cepacia) |
|---|---|
| Hemolysis | Negative (-ve) |
| Motility | Positive(+ve) |
| Nitrate Reduction | Negative (-ve) |
| Oxidase | Variable |
Is stenotrophomonas Maltophilia oxidase positive?
S. maltophilia is catalase-positive, oxidase-negative (which distinguishes it from most other members of the genus) and has a positive reaction for extracellular DNase. S. maltophilia is ubiquitous in aqueous environments, soil, and plants; it has also been used in biotechnology applications.
Is Burkholderia oxidase negative?
The organism is motile, indole negative, oxidase positive, inherently resistant to the polymyxins and gentamicin (3), and may produce a musty or earthy odor, although plates should not be sniffed due to the risk of laboratory exposure (30).
Where is Burkholderia cepacia complex found?
Burkholderia cepacia [burk-hōld–er–ee-uh si–pay–shee–uh] (also called B. cepacia) is the name for a group or “complex” of bacteria that can be found in soil and water. B. cepacia bacteria are often resistant to common antibiotics.
What organisms are in Burkholderia cepacia complex?
TABLE 1
| Species name originally assignedb | Burkholderia species name or taxon assigned | Reference |
|---|---|---|
| Pseudomonas cepacia | B. cepacia comb. nov. (B. cepacia genomovar I) | 81, 104, 119 |
| Pseudomonas solanacearum | B. solanacearum comb. nov. | 119 |
| Pseudomonas pickettii | B. picketti comb. nov. | 119 |
| Pseudomonas gladioli | B. gladioli comb. nov. | 119 |
Does Burkholderia grow on MacConkey agar?
Burkholderia mallei is also considered as a selective agent and causes glanders. Colonies can grow on MacConkey agar and selective media such as PC agar.
Is Stenotrophomonas oxidase negative?
Standard microbiology references describe Stenotrophomonas maltophilia as oxidase negative and variable with respect to utilization of lactose and sucrose.
Does Burkholderia cepacia grow on MacConkey agar?
Burkholderia cepacia organisms grow well on standard solid media such as 5% sheep blood, chocolate, and MacConkey agars; in thioglycollate and brain-heart infusion broths; and in broth-based blood culture systems.
Is Burkholderia cepacia Gram positive or negative?
gram-negative bacillus
Burkholderia cepacia is an aerobic gram-negative bacillus found in various aquatic environments. B cepacia is an organism of low virulence and is a frequent colonizer of fluids used in the hospital (eg, irrigation solutions, intravenous fluids). B cepacia rarely causes infection in healthy hosts.