What is BstEII?
Thermo Scientific Eco91I (BstEII) restriction enzyme recognizes G^GTNACC sites and cuts best at 37°C in O buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.
Are isoschizomers interchangeable?
Isoschizomers are interchangeable, and neoschizomers are not, because they cut differently even if they recognize the same sequence.
Is a type I restriction enzyme?
Type I. These restriction enzymes cut the DNA far from the recognition sequences. However, they do not produce discrete restriction fragments, hence, are of not much practical value. These are complex, multi-subunit restriction and modification enzymes.
Why are the restriction enzymes added last to the digestion mixtures?
The restriction enzyme is usually the last component added to a reaction to ensure that it is not exposed to extreme conditions. When many similar digests are being prepared, it may be convenient to create premixes of common reagents.
What is the difference between type I and type II restriction enzymes?
Restriction enzyme type 1 refers to a complex, multisubunit, combination of restriction-and-modification enzymes, which cut DNA at random far from their recognition sequences. Restriction enzyme type 2 refers to the enzymes, which cut DNA at defined positions close to or within their recognition sequences.
What should be included in Section 1 of Form I-9?
Section 1. Employee Information and Attestation (Employees must complete and sign Section 1 of Form I-9 no later than the first day of employment, but not before accepting a job offer.) Last Name (Family Name) First Name (Given Name) Middle Initial . Other Last Names Used (if any) Address (Street Number and Name) Apt. Number . City or Town . State
When to complete Form I-9 for Employment Eligibility Verification?
When completing Form I-9, Employment Eligibility Verification, you must make available to your employee the complete instructions to the form and the Lists of Acceptable Documents. Your newly hired employee must complete and sign Section 1 of Form I-9 no later than their first day of employment.
Who can complete the Spanish version of Form I-9?
Special Instructions Only employers and employees in Puerto Rico can complete the Spanish version of Form I-9. Spanish-speaking employers and employees in the 50 states and other U.S. territories may print this for their reference, but must complete the form in English to meet employment eligibility verification requirements.
Does an independent contractor need to complete Form I-9?
An independent contractor is not considered an employee for Form I-9 purposes and does not need to complete Form I-9.