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What type of test is the analytical profile index system?

What type of test is the analytical profile index system?

The analytical profile index or API is a classification of bacteria based on biochemical tests, allowing fast identification. This system is developed for quick identification of clinically relevant bacteria. Because of this, only known bacteria can be identified.

What is found in the API 20E profile index booklet?

In the API 20E, the plastic strip holds twenty mini-test chambers containing dehydrated media having chemically-defined compositions for each test. They usually detect enzymatic activity, mostly related to fermentation of carbohydrate or catabolism of proteins or amino acids by the inoculated organisms.

What is api20?

API 20 E is a standardized identification system for Enterobacteriaceae and other non-fastidious, Gram- negative rods which uses 21 miniaturized biochemical tests and a database.

What are API strips used for?

API strips give accurate identifications based on extensive databases and are standardized, easy-to-use test systems. The kits include strips that contain up to 20 miniature biochemical tests.

What is API 20ne used for?

This API-20E test strip (from bioMerieux, Inc.) is used to identify the enteric gram negative rods (although API makes a variety of other test strips for yeast, Staph, anaerobes, etc.) 20 separate test compartments are on the strip, all dehydrated. A bacterial suspension is used to rehydrate each of the wells.

What are enteric gram negative rods?

Gram negative enteric rods are rod-shaped bacteria that usually are found as single individual cells. This group is facultatively anaerobic, and includes the common enteric bacteria including many pathogens.

How do I read my EnteroPluri-test results?

Lay EnteroPluri-Test with its flat surface pointing upward and, by punching the plastic film, add 3 or 4 drops of Kovac’s Indole Reagent in the sector H2S/Indole. The reaction is positive if a pink-red colour develops in the added reagent within 10-15 seconds.

What is the API 20ne test?

The API 20 NE (BioMérieux) is a commercial biochemical identification system for gram-negative non-Enterobacteriaceae, such as Pasteurella. The test strip contains 20 miniature biochemical tests that are inoculated with a sample of a bacterial colony.

How can you tell if a rod is gram-negative?

Gram negative bacteria appear a pale reddish color when observed under a light microscope following Gram staining. This is because the structure of their cell wall is unable to retain the crystal violet stain so are colored only by the safranin counterstain.