What is a Calux assay?
This study demonstrates that the novel in vitro CALUX (chemical-activated luciferase expression) assay is a rapid, sensitive assay for assessing the toxic potency of (mixtures of) aryl hydrocarbon receptor (AhR)-active compounds in sediments and pore waters.
What is the meaning of CALUX?
Definition of calyx 1 : the usually green outer whorl of a flower consisting of separate or fused sepals. 2 : a cuplike division of the renal pelvis surrounding one or more renal papillae.
What is the principle of an assay?
A Gyrolab ready to use immunoassay kit is typically designed as a three-step sandwich assay. A biotinylated molecule is captured on streptavidin-coated beads in the affinity column in the CD. The sample binds to the biotinylated capture molecule.
What is Gamosepalous?
Gamosepalous refers to a flower that possesses a calyx, consisting of fused sepals. E.g. Hibiscus. Polysepalous refers to a flower that possesses a calyx, consisting of separate sepals.
How many types of assay are there?
Assays can be divided into three main categories based on the type of sample used – ligand-binding assays that measure binding between a ligand and a receptor, immunoassays that detect antibody-antigen binding, and bioassays that measure biological activity in response to certain stimuli.
What is luciferase assay?
A luciferase assay is used to determine if a protein can activate or repress the expression of a target gene.
How does luciferase assay work?
A Luciferase Reporter Assay. When this protein activates transcription, the cell will produce luciferase enzyme. After the addition of a lysis buffer and a substrate, a luminometer quantifies the luciferase activity. If your protein activates the expression of the target gene, the amount of signal produced increases.
What is meant by Polysepalous and Gamosepalous?
Gamosepalous refers to a flower that possesses a calyx, consisting of fused sepals. E.g. Hibiscus. Polysepalous refers to a flower that possesses a calyx, consisting of separate sepals. E.g. Rose.
What is Polypetalous and Gamopetalous?
Gamopetalous: Flowers with fused petals; examples are Bindweed and Elderberry. Polypetalous: Flowers with free petals; examples are Rose and Camellia.
What is calyx and its functions?
The outer whorl of a flower composed of sepals is known as calyx. It protects the inner parts of the flower during development.
What is the calyx made up of?
sepals
flower parts: (1) an outer calyx consisting of sepals; within it lies (2) the corolla, consisting of petals; (3) the androecium, or group of stamens; and in the centre is (4) the gynoecium, consisting of the pistils.
What are the two main types of assay?
The main types of assay used for blood screening are: Immunoassays (IAs): — Enzyme immunoassays (EIAs)…Enzyme immunoassays (EIAs) and chemiluminescent immunoassays (CLIAs)
- Base and sides of a polystyrene microwell.
- Surface of polystyrene or other material.
- Micro-particles.
What are different types of assay?
The main types of assay used for blood screening are:
- Immunoassays (IAs): — Enzyme immunoassays (EIAs) — Chemiluminescent immunoassays (CLIAs) — Haemagglutination (HA)/particle agglutination (PA) assays. — Rapid/simple single-use assays (rapid tests)
- Nucleic acid amplification technology (NAT) assays.
What is the role of luciferase?
A. Luciferase enzymes (firefly, Renilla, or Gaussia) expressed in the cytoplasm of engineered cells catalyze production of light photons from the appropriate substrate. Beetle luciferases react with luciferin in a chemical reaction that requires magnesium, oxygen, and ATP.
What is luciferin used for?
Luciferin is widely used in science and medicine as a method of in vivo imaging, using living organisms to non-invasively detect images and in molecular imaging. The reaction between Luciferin substrate paired with the receptor enzyme Luciferase produces a catalytic reaction, generating bioluminesce.
What is the purpose of the luciferase?
The power of luciferase has been harnessed by scientists to devise reactions whose light output is used to monitor biological processes including gene expression, biomolecular binding, and cell viability.
How can CALUX® assays be used to detect certain chemicals?
The CALUX® (Chemical Activated Luciferase gene eXpression) Assays from BioDetection Systems can be used to detect certain chemicals like dioxins, dioxin-like PCB’s or (anti)estrogen and (anti)androgen compounds. The innovative CALUX® cells have been tailored so that they produce light in a dose-responsive way when exposed to the chemicals.
What is CALUX used for?
Chemical Activated LUciferase gene eXpression (CALUX) is a bioassay used in the detection of specific chemicals in samples. It consists of a modified cell line that has a DNA construct with a luciferase reporter gene in conjunction with response elements that can induce transcription of the inserted gene for the light-generating enzyme.
How selective is the CALUX assay for androgens?
The CALUX assay was found to be selective for androgens and seemed not influenced by signaling through other steroid receptors. Copyright 2010 Elsevier Inc. All rights reserved. Androgen Antagonists / pharmacology*
What does AR-CALUX stand for?
Environmental chemicals having sex hormone-like activity are detected by similar bioassays as well, including the Estrogen Receptor-Responsive CALUX (ER-CALUX) and Androgen Receptor-Responsive CALUX (AR-CALUX). ^ Murk, A.J.; J. Legler; M.S. Denison; J.P. Giesy; C. van de Guchte; A. Brouwer (1 September 1996).