What is tetramethylbenzidine used for?
3,3′,5,5′-Tetramethylbenzidine or TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA).
What is the composition of tetramethyl benzidine reagent?
The main component for this poroduct is 3,3′,5,5′-Tetramethylbenzidine, TMB, it was used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product. The density of blue is proportional to HRP activity.
What is tetramethylbenzidine test?
The tetramethylbenzidine (TMB) presumptive test for blood is a catalytic test which is based on the peroxidase-like activity of hemoglobin.
Is TMB toxic?
Harmful by inhalation, in contact with skin and if swallowed. Irritating to eyes, respiratory system and skin. Very toxic to aquatic organisms, may cause long-term adverse effects in the aquatic environment.
Why is TMB used in ELISA?
Background: TMB (3,3′, 5,5”-tetramethylbenzidine) is used as a substrate to generate detectable signal in ELISA. The reaction between the TMB substrate and a peroxidase, typically horseradish peroxidase (HRP), produces a measur- able color change that correlates with analyte level.
How do you make tetramethylbenzidine solution?
Dissolve 1 g of 3,3′,5,5′-tetramethylbenzidine in 90 ml of glacial acetic acid and make up to 100 ml with water. The solution will keep for several weeks in a dark bottle at 4°C. Dilute 1 volume of 3% (‘10 vols’) H 2 O 2 with 2 volumes of water before use. Use 100 g/l glacial acetic acid.
What is the concentration of diaminobenzidine tetrahydrochloride in Tris-HCl?
3,3′-Diaminobenzidine tetrahydrochloride (5 mg/ml) in 100 m M Tris-HCl, pH 7.6. [ Note: store frozen at –15°C for up to 1 year without loss of activity]
How do you store diaminobenzidine tetrahydrochloride?
Diaminobenzidine reaction product is stable in commonly used buffers and organic solvents. 3,3′-Diaminobenzidine tetrahydrochloride (5 mg/ml) in 100 m M Tris-HCl, pH 7.6. [ Note: store frozen at –15°C for up to 1 year without loss of activity]
How does imidazole-intensified diaminobenzidine react with Em?
Imidazole-intensified diaminobenzidine gives an amber-brown amorphous reaction product by LM, an amorphous electron-dense reaction product by EM. Warning: 3,3′-Diaminobenzidine (DAB) is a potential carcinogen. Wear gloves while handling. After reaction, DAB should be disposed of carefully.