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What are HEK 293 cells used for?

What are HEK 293 cells used for?

HEK293 are frequently used for heterologous expression of cell membrane receptors and ion channels, and many studies have utilized this cell line to investigate the signalling pathways of G protein-coupled receptors (GPCRs) which are implicated in disease.

What is the difference between 293 and 293T cells?

The key difference between HEK293 and HEK293t is that HEK293 is an immortalized cell line derived from the embryonic human kidney that is transfected with sheared human adenovirus type 5DNA while HEK293t is a daughter cell line derived from HEK293 original cell line that is transfected with a plasmid vector carrying …

Where did 293T cells come from?

The 293T cell line was created in Michele Calos’s lab at Stanford by stable transfection of the HEK 293 cell line with a plasmid encoding a temperature-sensitive mutant of the SV40 large T antigen; it was originally referred to as 293/tsA1609neo.

Why is HEK293 used for transfection?

Advantages of HEK293 cells Firstly, they are very easy to grow and to maintain, with high reproducibility, which makes them preferable over other less-robust and slow-growing cell lines. Furthermore, they are very efficient at protein production and accessible for transfection.

What is the T in 293t?

Macquarie University. Basically HEK293 cells are the parental line for HEK293T. Here the letter “T” implies that it expresses the Large T antigen that is essential for replicating plasmids containing an SV40 origin of replication to high copy number in the transfected cell. Cite.

What is a 293T cell?

The 293T cell line, originally referred as 293tsA1609neo, is a highly transfectable derivative of human embryonic kidney 293 cells, and contains the SV40 T-antigen. Age fetus Comments. This cell line is competent to replicate vectors carrying the SV40 region of replication.

How do 293T cells grow?

Place the cells in the 37 °C incubator with 5% CO2. 6. Gently replace medium after 24 hours with 14 mL HEK293T Culture Medium and continue to culture at 37 ºC with 5% CO2. Cells should be ready for passage or expansion to a T175 flask in ~ 2-3 days.

What is ATCC full form?

2The American Type Culture Collection. The American Type Culture Collection (ATCC) is a private, nonprofit organization dedicated to the acquisition, preservation, authentication, and distribution—the “APAD” activities—of diverse biological materials.

Why are HEK293 cells immortal?

HEK 293 immortal cell line that is comparatively easy to handle. An immortalised cell line is a population of cells from a multicellular organism which would normally not proliferate indefinitely but, due to mutation, have evaded normal cellular senescence and instead can keep undergoing division.

What type of cell is HEK293?

Originating from a female fetus, HEK293 cells are today among the most used mammalian cell lines for a wide range of applications because of their ease of transfection as well as culture. Despite stemming from kidney tissue, HEK293 cells – time and time again – exhibited properties of immature neurons.

Is HEK 293 cancerous?

Thus, HEK293 is not a model for normal human cells; these cells are immortalized already by known oncogene but not malignant yet.