What is the wavelength for Cy5?
Invitrogen Cy5 dye is a bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines.
What color does Cy3 fluorescence?
orange-
Cy3 dye dashboard The Cy3 dye is a traditional orange-fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications.
What Cy5 5?
Cy5. 5 is a far-red (and near-infrared) emitting dye which is ideal for fluorescence measurements where background fluorescence is a concern. It is also suitable for in vivo NIR imaging experiments.
What is a FAM dye?
Fluorescein amidites, abbreviated as FAM, are important synthetic equivalents of fluorescein dye used in oligonucleotide synthesis and molecular biology.
What is Cy5?
Cy5 dye is a traditional far-red–fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications. It is also the basis for the classic lipophilic tracer DiD and its variants.
What color is cy2?
Cyanine, Cy™2 Technical Information Cy™2 – conjugates have maximum absorption/excitation at 492 nm and fluoresce with a peak around 510 nm in the green region of the visible spectrum like FITC conjugates (520 nm), but they are more photostable and less sensitive to pH changes than FITC.
Is FITC a fluorescent dye?
Fluorescein-5-isothiocyanate (FITC) is a fluorescence dye and belongs to the xanthene dyes. FITC is used for labeling of different biomolecules, e.g. immunoglobulins, lectins and other proteins, peptides, nucleic acids, nucleotides; oligo-and polysaccha- rides.
What is the size of Cy3?
Cy3 fluoresces greenish yellow (~550 nm excitation, ~570 nm emission), while Cy5 is fluorescent in the far-red region (~650 excitation, 670 nm emission).
What color is Tritc?
Tetramethylrhodamine (TRITC) is a bright orange-fluorescent dye with excitation ideally suited to the 532 nm laser line. It is commonly conjugated to antibodies and proteins for cellular imaging applications.
What is Cy3 and Cy5?
Cy3 fluoresces greenish yellow (~550 nm excitation, ~570 nm emission), while Cy5 is fluorescent in the far-red region (~650 excitation, 670 nm emission). Cy3 can be detected by various fluorometers, imagers, and microscopes with standard filters for Tetramethylrhodamine (TRITC).
What color is Alexa Fluor 750?
red
Alexa Fluor® 750 (AF750, Alexa 750) is a far-red emitting synthetic fluorophore that can be excited by the 640 nm red laser and captured with a 780/60 nm bandpass filter.
Is Cy5 far-red?
The far-red dyes Cy5 and Alexa 647 are popular choices for diverse fluorescence applications because they have large extinction coefficients, high quantum yields, and excitation maxima at frequencies with low cellular absorption.
Does Cy5 do Photobleach?
Cyanine-3 (Cy3) and cyanine-5 (Cy5) are ubiquitously used for nucleotide labeling. In previous works, we showed that they possess a varying degree of susceptibility toward photobleaching [31]. …
Can you use APC and PE Cy5 together?
For example, PE-Cy™5 is very bright with regard to stain index (Table 1), but it has considerable spillover (ie, adds background) into the APC detector. While these two fluorochromes can be used together, the resolution sensitivity in APC will be reduced compared to, for example, a combination of PerCP-Cy5. 5 and APC.
Is Cy5 a protein?
The Cy5-labeled protein has excitation and emission wavelengths at 650 nm and 670 nm respectively, and can be directly used for downstream applications including ELISA, western blot, Immunohistochemistry, Immunofluorescence, and FACS, etc.