What are MCF-7 cell lines?
1 MCF-7 human adenocarcinoma cells. MCF-7 is a human breast cancer cell line with estrogen, progesterone and glucocorticoid receptors [26]. It is derived from the pleural effusion of a 69-year-old Caucasian metastatic breast cancer (adenocarcinoma) in 1970 by Dr Soule of the Michigan Cancer Foundation, Detroit, MI [27] …
How do you split MCF-7 cells?
Until the cells become well attached, MCF7 cells should be treated as a mixed adherent and suspension cell line. Simply add fresh media as the size and number of cell clusters increase. Clusters of cells in suspension should be broken apart by gently pipetting with a small bore pipette (5 mL or smaller).
What does the acronym MCF-7 represent?
MCF-7 is a breast cancer cell line isolated in 1970 from a 69-year-old White woman. MCF-7 is the acronym of Michigan Cancer Foundation-7, referring to the institute in Detroit where the cell line was established in 1973 by Herbert Soule and co-workers.
What are MCF-7 ADR cells?
Abstract. MCF-7/ADR cells have been widely used as a multidrug-resistant breast cancer cell model in cancer research. The origin of MCF-7/ADR has been a matter of debate since MCF-7/ADR cells were re-designated NCI/ADR-RES in 1998.
What is the doubling time of MCF-7 cells?
MCF-7 human mammary carcinoma cells were inoculated into 150-sq cm flasks at 3 X 10(5) cells/flask, and after a lag period of about 48 hr, these cells grew exponentially for 5 days with a mean population doubling time of about 24 hr.
Is MCF-7 a gene?
In microarray profiles, the MCF-7 gene set clusters with the luminal subtype of breast cancer. MCF-7 xenografts express wild-type p53 and are nonmetastatic even when implanted orthotopically into the mammary fat pad.
Are MCF-7 cells adherent?
MCF-7 is an adherent, epithelial luminal cell line positive for estrogen and progesterone receptors. Although they are referred to as hormone-dependent, the minimal amount of steroid hormones present in serum is sufficient to ensure cell growth.
Does MCF-7 have HER2?
Two particularly important members of this large family are the epidermal growth factor receptor (EGFR), which is activated by the epidermal growth factor (EGF), and the human epidermal growth factor receptor-2 (HER2), both present in MCF-7 cells (7).
Are MCF-7 cells ER positive?
With time, MCF-7 has produced more data of practical knowledge for patient care than any other breast cancer cell line (4) (Table I). It is ER-positive and progesterone receptor (PR)-positive (8) and belongs to the luminal A molecular subtype (2).
Does MCF-7 express HER2?
MCF7-HER2 cells, treated with an inhibitor of protein synthesis cycloheximide show a slower decrease in survivin protein level in HER-2 over-expressing MCF7 cells than in control cells (data not shown), which suggests that up-regulation of survivin is post translational.