What does perlecan do?
Perlecan is especially important in corneal epithelial development (Inomata et al., 2012) and maintenance of basement membrane integrity in regions with high mechanical stress, including the contracting myocardium and expanding brain vesicles (Costell et al., 1999).
Is perlecan a protein?
Perlecan (PLC) also known as basement membrane-specific heparan sulfate proteoglycan core protein (HSPG) or heparan sulfate proteoglycan 2 (HSPG2), is a protein that in humans is encoded by the HSPG2 gene.
Is perlecan a glycoprotein?
Perlecan protein core interacts with extracellular matrix protein 1 (ECM1), a glycoprotein involved in bone formation and angiogenesis. J Biol Chem. 2003 May 9;278(19):17491-9. doi: 10.1074/jbc.
Is Perlecan a proteoglycan?
Perlecan is one of the largest proteoglycans discovered, possessing a protein core of approximately 500 kDa that can be modified by the addition of N-terminal heparan sulfate (HS) side chains, measuring ~65 kDa each [37].
Where is Perlecan found?
mesenchymal organs
Perlecan is generally found in mesenchymal organs and connective tissues (Handler et al., 1997). The 470-kDa core protein, of which only a single form has been identified, displays five distinct polypeptide domains. Domain I contains three and domain V contains one potential attachment site for HS or CS chains.
Is fibrillin a collagen?
Fibrillin is a glycoprotein, which is essential for the formation of elastic fibers found in connective tissue….Fibrillin.
| fibrillin 1 | |
|---|---|
| NCBI gene | 2200 |
| HGNC | 3603 |
| OMIM | 134797 |
| PDB | 2W86 |
Why is fibrillin-1 Important?
In this matrix, molecules of fibrillin-1 attach (bind) to each other and to other proteins to form threadlike filaments called microfibrils. Microfibrils form elastic fibers, which enable the skin, ligaments, and blood vessels to stretch.
How many types of GAGs are there?
The four primary groups of GAGs are classified based on their core disaccharide units and include heparin/heparan sulfate, chondroitin sulfate/dermatan sulfate, keratan sulfate, and hyaluronic acid.
Is Aggrecan extracellular matrix?
Aggrecan-based extracellular matrix is an integral part of the human basal ganglia circuit. Neuroscience. 2008 Jan 24;151(2):489-504. doi: 10.1016/j.
Is Aggrecan a GAG?
Almost 90% of aggrecan mass is comprised of substituted GAG chains which are mostly chondroitin sulfate chains, but also include keratan sulfate chains with N- and O-linked oligosaccharides.
What is the origin of perlecan?
Perlecan was originally isolated from a tumor cell line and shown to be present in all native basement membranes. Perlecan is a large multidomain (five domains, labeled I-V) proteoglycan that binds to and cross-links many extracellular matrix (ECM) components and cell-surface molecules.
What is the function of perlecan?
Perlecan is a large multidomain (five domains, labeled I-V) proteoglycan that binds to and cross-links many extracellular matrix (ECM) components and cell-surface molecules. Perlecan is synthesized by both vascular endothelial and smooth muscle cells and deposited in the extracellular matrix.
What is the molecular weight of perlecan?
Perlecan consists of a core protein of molecular weight 470 kDa to which three long chains (each approximately 70-100 kDa) of glycosaminoglycans (often heparan sulfate, HS, but can be chondroitin sulfate, CS) are attached. The core protein consists of five distinct structural domains.
Is perlecan similar to laminin A?
“The complete sequence of perlecan, a basement membrane heparan sulfate proteoglycan, reveals extensive similarity with laminin A chain, low density lipoprotein-receptor, and the neural cell adhesion molecule”. The Journal of Biological Chemistry. 266 (34): 22939–47. doi: 10.1016/S0021-9258 (18)54445-8. PMID 1744087.