Is Zeatin water soluble?
Solubility : Soluble in water, DMF (0.5 mg/ml), alcohol , DMSO (3 mg/ml), 1:1 solution of DMSO:PBS (pH 7.2) (0.5 mg/ml), aqueous alcohol (50 mg/ml), and glacial acetic acid (50 mg/ml).
What is the function of Zeatin?
Zeatin is a cytokinin derived from adenine, which occurs in the form of a cis- and a trans-isomer and conjugates. Zeatin was discovered in immature corn kernels from the genus Zea. It promotes growth of lateral buds and when sprayed on meristems stimulates cell division to produce bushier plants.
Is Zeatin naturally occurring?
In the presence of auxin (but not in its absence) kinetin stimulated tobacco pith parenchyma tissue to proliferate in culture. Later, zeatin was identified as the first naturally occurring cytokinin in immature maize endosperm (Letham, 1973), and turns out to be the abundant cytokinin in coconut milk.
What can you say about zeatin?
Zeatin (0.05 mg/liter) was used as a component of the nutrient media for cultivation of flavan-synthesizing tissues and cells of Theobroma cacao, though the specificity of its effect was not studied (Jalal and Collin, 1977).
What is kinetin and zeatin?
Kinetin is the active form of zeatinZeatin is the active form of kinetinZeatin is a synthetic cytokinin and kinetin is naturally occuring Zeatin is a naturally occuring plant cytokinin, kinetin is not. Answer : D. Loading Books.
What is the source of zeatin?
Zeatin is derived from adenine and is a plant hormone. It is a cytokinin and was discovered in immature corn kernels.
How do you dissolve kinetin?
Dissolve 21.5 mg of Kinetin (GoldBio Catalog # K-100) in 1 ml of 1N NaOH. 2. Bring solution to 10 ml with molecular biology grade water to get a final solution of 10mM.
How do you dissolve cytokinin?
This synthetic cytokinin should be dissolved in 0.01‐M or 0.1‐M hydrochloric acid or sodium hydroxide in water or 70% ethanol. The latter is preferable because it is also a sterilant.
Who discovered zeatin?
In searching for naturally occurring cytokinins in plant tissues, Letham in 1963 isolated a cytokinin, zeatin, from immature corn kernels.
Is kinetin soluble in water?
Kinetin Properties Soluble in water (<1 mg/ml) at 25°C, Acetic acid (49.00-51.00 mg/ml), DMSO (43 mg/ml) at 25°C, ethanol (<1 mg/ml) at 25°C, 0.1 N Sodium hydroxide (1% w/v), methanol (slightly), and dilute aqueous hydrochloric acid or sodium hydroxide (freely).
What is the difference between kinetin and Zeatin?
The first cytokinin was discovered as kinetin (A modified form of adenine). Kinetin does not occur naturally in plants. Search for natural substances with cytokinin like activity led to the isolation of zeatin from corn-kernels and coconut milk.
Can Zeatin be autoclaved?
All of these adenine-based cytokinins showed exceptional stability following an autoclave cycle at 121°C, 110 kPa for 30 min when in solutions of 1.0 mg mL−1 in 0.05 N KOH, with no significant degradation detected. Trans-zeatin was also found to be stable after one autoclave cycle with 1× MS-basal salts.
What is the source for isolating of zeatin?
The hormone was isolated from the corn kernels and the coconut milk, where the hormone was responsible for cytokinesis.
What is zeatin plant?
May 19, 2014. Zeatin is a plant growth hormone in the cytokinin family. It consists of a purine ring structure with a side chain that contains hydroxyl, amino, and olefin groups. It was discovered in the corn genus Zea, for which it is named. Zeatin and other cytokinins are produced in plants’ root systems.
How do you dissolve Thidiazuron?
Thidiazuron is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF), which should be purged with an inert gas. The solubility of thidiazuron in these solvents is approximately 1, 15, and 30 mg/ml, respectively.
How do you dissolve a BAP?
Benzyladenine (6‐benzylaminopurine, N6‐benzylaminopurine, BA or BAP) can be dissolved in 0.1‐M hydrochloric acid or 95 or 70% ethanol. It is best to dissolve it and other cytokinins in 70% ethanol because the alcohol is also a ster‑ ilant.
How do you make Thidiazuron stock solution?
Dissolve TDZ in 2-3 ml of either KOH or NaOH. Then make final volume by adding glass distilled water. To make 10 ml of 1mg/ml TDZ solution, I dissolved 10 mg (0.02 g) of TDZ in 1ml of 1N NaOH, then adjusted to 10ml with distilled water.