What is FuGENE transfection reagent?
The FuGENE® HD Transfection Reagent is a nonliposomal formulation designed to transfect DNA into a wide variety of cell lines with high efficiency and low toxicity.
How do you use FuGENE?
Briefly vortex or mix the FuGENE® HD Transfection Reagent/DNA complex. Add 2–10µl of complex per well to 96-well plate containing 100µl of cells in growth medium. Mix by pipetting or using a plate shaker for 10–30 seconds. Return plates to the incubator.
Is FuGENE toxic to cells?
Fugene was found to be the most toxic reagent for this cell line, with a viability of 68.45%.
How does FuGENE HD work?
FuGENE® HD interacts with nucleic acids & the cell’s membrane to provide efficient and safe entry into the cell. This mechanism allows users to overcome barriers in difficult-to-transfect lines such as primary cells, stem cells, and suspension cells, and has successfully been used to transfect over 1000 cell lines.
Is FuGENE lipid based?
The FuGENE HD Transfection Reagent is lipid-based, simple-to-use, and can result in high transfection efficiencies with minimal cyototoxicity.
What is in FuGENE 6 transfection reagent?
For life science research only. Not for use in diagnostic procedures. FuGENE® 6 Transfection Reagent is a proprietary blend of lipids and other components supplied in 80% ethanol, sterile-filtered, and pack- aged in glass vials.
What is FuGene HD transfection reagent?
FuGENE® HD Transfection Reagent lets you work in your cancer model. Use the FuGENE® HD protocol database to see if transfection conditions for your cell line are available. The database includes over 25 cancer models and provides transfection protocols specific for each cell line.
What is the Nucleofector device?
The Nucleofector TM Device is the single cuvette based system that has been used in research labs since 2001. It allows efficient transfection of hard-to-transfect cell lines and primary cells with different substrates (e.g., DNA vectors or siRNA oligonucleotides) in low-throughput format.
What is transfection?
Transfection of hard-to-transfect cells, including primary cells, stem cells, neurons and cell lines, as well as cells in adherence.
Can the Nucleofector TM 2B device be used for bacteria transformation?
The Nucleofector TM 2b Device (or older generations I or II) can also be used for bacteria transformation by using alternative cuvettes. There are cell type-specific Optimized Protocols or recommendations available in our knowledge database.
FuGENE® HD interacts with nucleic acids to provide efficient and safe entry into the cell, allowing users to overcome barriers in difficult-to-transfect lines such as primary cells, stem cells, and suspension cells. Its simple, easy-to-use protocol allows users to free up valuable lab time.
What is the difference between stable and transient transfection?
In stable transfection, the plasmid DNA successfully integrates into the cellular genome and will be passed on to future generations of the cell. However, in transient transfection, the transfected material enters the cell but does not get integrated into the cellular genome.
Is fugene toxic to cells?